IN281595: A set of oligonucleotide primers useful for the species specific detection of plasmodium species
Abstract
The invention provides for highly specific and sensitive nested Polymerase Chain Reaction (PCR) method for detecting the presence or absence of Plasmodium falciparum and Plasmodium vivax in clinical specimens. This method targets a 286bp region of the Plasmodium falciparum and 294bp region of the Plasmodium vivax 28S Ribosomal RNA gene. Nucleotide sequences for highly specific novel primers derived from this 28S rRNA gene are also disclosed. These primers are used with the nested polymerase chain reaction method to amplify targeted nucleotide sequences within the ~790 basepair segment of the 28S Ribosomal RNA gene. This approach facilitates the application of Nested PCR to P.falciparum and P.vivax detection in lab settings. The novel primers and optimized reaction conditions of the nested Polymerase Chain Reaction method enable significantly greater sensitivity for the detection of Plasmodium falciparum and Plasmodium vivax DNA in tissue suspected of harboring the respective pathogen and thus helpful in detecting mixed infections.
Filing Date:
31-03-2009
Issue Date:
21-03-2017
Applicant:
CSIR(SCH)
Patent No:
IN281595
Application No:
0659DEL2009
Inventor(s):
Ashis Das, Deepak Pakalapati
IPC Classification:
C12Q
Country:
India
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