Patents

The invention provides a composition and process for removal of colours and inhibitors from tissues to isolate RNA. Particularly, this invention relates to a composition and the method to remove the soluble substances that otherwise would impart colour to the RNA preparations or would inhibit down stream applications including synthesis of complementary DNA (cDNA) and polymerase chain reaction using RNA as one of the substrates. Reported protocols till date can isolate RNA, but the isolated RNA is not suitable for downstream processes such as RT-PCR due to presence of colours and the associated interfering agents. None of the protocols describe a simple and quick method for removal of these interfering chemicals.