Characterization of 500 isolates were completed. Transcriptional expression analysis of mrcA, mrcB and mtgA was performed by Real- Time PCR S.N. Cloning of full-length OXA-10, OXA-23 and VEB1 genes in araBAD-induced pBAD-18 CAM vector is done. OXA-20 and VEB-1 were constructed by gene synthesis. Antibiotic susceptibility assay and Biofilm formation upon in vivo expression of OXA- 10 and OXA-23 beta-lactamase are done.Cloning, Expression, Purification, and Enzyme kinetic analysis of OXA-10, OXA- 20 and OXA-23 beta-lactamase is performed.
Assam University, Silchar, Assam
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