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Designing a secondary resistance-based biomarker for detection of antimicrobial resistance (AMR) in multi drug resistant bacteria

Implementing Organization

Assam University
Principal Investigator
Amitabha Bhattacharjee
Assam University, Silchar, Assam
ab0404@gmail.com
Principal Investigator
Dr. Anindya Sundar Ghosh
Indian Institute of Technology (IIT)Kharagpur, West Bengal
asghosh@bt.iitkgp.ac.in
CO-Principal Investigator
Prof. Manabendra Dutta Choudhury
Assam (Central) University, Silchar, Assam
drmdc@bioinfoaus.ac.in

Project Overview

In the proposed study based on in silico gene network analysis, associated secondary resistance genes will be studied for their transcriptional expression using antibiotic as signal molecule. Laboratory strain, clinical isolates, mutants and clones will be used in the study. mRNA sequence of secondary resistance genes will be done to understand the sequence variation of over expressed one from normal one. A quantitative PCR based assay will be developed to detect multidrug resistance using secondary resistome as biomarker which will be simple and a reliable tool in diagnostic microbiology laboratory.

Achievements

Characterization of 500 isolates were completed. Transcriptional expression analysis of mrcA, mrcB and mtgA was performed by Real- Time PCR S.N. Cloning of full-length OXA-10, OXA-23 and VEB1 genes in araBAD-induced pBAD-18 CAM vector is done. OXA-20 and VEB-1 were constructed by gene synthesis. Antibiotic susceptibility assay and Biofilm formation upon in vivo expression of OXA- 10 and OXA-23 beta-lactamase are done.Cloning, Expression, Purification, and Enzyme kinetic analysis of OXA-10, OXA- 20 and OXA-23 beta-lactamase is performed.

Source

Source
E-promis and Information received by Investigator

Related Research

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Funding Organization
Funding Organization
Department of Science and Technology (DBT)
Quick Information
Area of Research
Medical Sciences
Focus Area
Antimicrobial Resistance, Diagnostics
Start Date
21 Sep 2023
End Date
20 Sep 2026
Status
ongoing
Email
ab0404@gmail.com
Output
No. of Research Paper
00
Technologies (If Any)
00
No. of PhD Produced
00
Publications
01
No. of Patents
Filed : 00
Grant : 00
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