The project involved collecting fecal samples from diverse wildlife and domestic animals across Kashmir, Ladakh, and northeastern India, testing for Mycobacterium avium subspecies paratuberculosis (MAP). Samples underwent staining, PCR, culture, and antibody detection, revealing MAP prevalence of 37.8% via microscopy and PCR, with the Bison strain circulating among sheep and goats. Fecal The project involved collecting fecal samples from diverse wildlife and domestic animals across Kashmir, Ladakh, and northeastern India, testing for Mycobacterium avium subspecies paratuberculosis (MAP). Samples underwent staining, PCR, culture, and antibody detection, revealing MAP prevalence of 37.8% via microscopy and PCR, with the Bison strain circulating among sheep and goats.