Twenty grams of dried tea leaves were infused in 200 ml boiling water for 5min, after which the leaves were filtered out. The resulting infusion contained catechins along with other water-soluble compounds and pigments. To remove caffeine and other pigments, the infusion was extracted with dichloromethane. The aqueous layer, containing catechins, was then subjected to ethyl acetate extraction to obtain the catechin fraction. Twenty grams of dried tea leaves were infused in 200 ml boiling water for 5min, after which the leaves were filtered out. The resulting infusion contained catechins along with other water-soluble compounds and pigments. To remove caffeine and other pigments, the infusion was extracted with dichloromethane. The aqueous layer, containing catechins, was then subjected to ethyl acetate extraction to obtain the catechin fraction.