Research Projects

The aim is to develop the bioengineered corneal organoid to mimic the natural functional characteristics, along with miRNA (miR-146a) delivery to reduce inflammation and aid in corneal regeneration in diseased conditions. Gelatin is a biocompatible, thermo-reversible polymer with shear-thinning properties and mimics the collagen composition, which contains RGD peptides that facilitate cell attachment. Carboxymethyl cellulose is a biocompatible and transparent polymer (refractive index is an important parameter in the corneal studies), which provides mechanical and structural stability. This novel combination of CMC and gelatin mimics the ECM components and maintains the mechanical properties and refractive index critical for corneal function. It facilitates limbal stem cells attachment and differentiation and aids in the regenerative capacity of corneal organoids under in vitro conditions. Additionally, conjugation of miR-146a facilitates cellular uptake, resulting in the sustained release and reducing the inflammation for a longer duration. The developed bioengineered corneal organoid is characterized using a variety of techniques and validated through in vitro studies. Further, comparison of organoids with in vivo rat models helps to understand the regeneration process and inflammation reduction, so that organoids may reduce or replace animal studies.