Research Projects

This project aims to develop 3D in vitro models using human normal and cystic ovarian tissues to mimic the ovarian niche, enabling the study of cumulus–oocyte interactions and improving IVM protocols for better fertility outcomes. Ovarian cysts are a highly prevalent gynaecological condition that disrupt the delicate balance of female reproductive physiology, primarily by impairing the maturation of oocytes, a critical process for successful fertilization and pregnancy. These cystic conditions contribute to subfertility and present significant challenges to the effectiveness of assisted reproductive technologies (ART), particularly in patients undergoing in vitro fertilization (IVF) or related interventions. To address this unmet clinical need, the present project proposes an innovative translational approach that bridges clinical observations with advanced laboratory techniques, aiming to develop and utilize three-dimensional (3D) cumulus cell culture systems derived from human ovarian tissue, both normal and cystic. By engineering 3D culture platforms that replicate the ovarian microenvironment, the study seeks to create physiologically relevant models that support cell-cell communication, hormonal responsiveness, and metabolic exchanges necessary for oocyte development and maturation. Cumulus cells will be carefully isolated from ovarian samples obtained from patients, verified through specific molecular markers for identity and viability, and embedded into bioengineered 3D scaffolds that mimic in vivo structural and functional conditions. These culture systems will be optimized with key hormonal and nutritional components to closely simulate follicular dynamics, thereby providing a robust platform for co-culturing immature oocytes and assessing their potential to undergo in vitro maturation (IVM). The performance of these systems will be evaluated by comparing the maturation outcomes of oocytes cultured in normal versus cystic cumulus cell environments, with a focus on identifying cellular behaviours and molecular signals that differ across conditions. Advanced transcriptomic and proteomic analyses will uncover disrupted pathways linked to impaired oocyte maturation in cystic ovaries, enhancing understanding of cumulus–oocyte interactions and guiding the development of personalized IVM protocols. This integrative study merges state-of-the-art 3D culture technologies with high-throughput molecular analysis and clinical relevance, ultimately aiming to advance fertility preservation strategies and optimize ART outcomes. By targeting the root biological causes of oocyte dysfunction in cystic ovaries, this research promises to contribute significantly to the future of reproductive medicine and the development of innovative, patient-specific therapeutic approaches.