Research Projects

Ten potential ligands of CAMKIV were suggested by docking simulation studies, were successfully synthesized and characterized. All ten molecules bind efficiently to the active site of CAMKIV. Molecules 1 to 8 can bind to CAMKIV with stoichiometry ratio of 1:1, however, molecule 9 and 10 in stoichiometry ratio of 2:1 and 3:1, respectively. The CAMKIV–ligand complex is stabilized by several non-covalent interactions offered by residues in vicinity to the active site. We further observed that molecule 10 is showing best anticancer activity on HuH7 cell line. Propidium iodide (PI) staining and flow cytometry were used to determine the cell apoptosis and cell cycle stage analysis. At the different concentration of molecules, we analyzed the percent apoptotic inhibition. Out of ten molecules, molecule 10 shows the best apoptotic potentials which are an excellent agreement with the MTT assay and fluorescence binding study of molecule 10