Development of RNA aptamer conjugated nanoparticle-5hmdC and 5fdC targeting cytidine deaminase expressing cancer cells
Implementing Organization
National Institute Of Pharmaceutical Education And Research (NIPER), Mohali
Principal Investigator
Dr. Gaurav Parashar
National Institute Of Pharmaceutical Education And Research (NIPER), Mohali
CO-Principal Investigator
Dr. K B Tikoo
Professor
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National Institute Of Pharmaceutical Education And Research (NIPER), Mohali
Project Overview
Cloning, expression and purification of cytidine deaminase (CDA). CDA specific internalising RNA aptamer isolation using systemic evolution of ligands by exponential enrichment (SELEX), functional validation of enriched CDA specific aptamer in differentially expressing cell lines, synthesis of PLGA-PEG-COOH nanoparticle-aptamer conjugate containing modified nucleotides (5hmdC and 5fdC), and invitro and invivo evaluation of nanoparticle-aptamer conjugate for targeted delivery of 5hmdC and 5fdC and downstream effect on cancer proliferation
National Institute Of Pharmaceutical Education And Research (NIPER), Mohali
Professor
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