Understanding the role of SARS-CoV-2 virulence factor-Orf3a in modulating host cell responses: A way to identify host-directed therapy

Acces sibility Controls

Implementing Organization

CSIR-Institute of Microbial Technology (CSIR-IMTECH), Chandigarh

Principal Investigator

Dr Amit Tuli

Scientist

CSIR-Institute of Microbial Technology (CSIR-IMTECH), Chandigarh

About

The aim of this grant application is to understand the role of SARS-CoV-2 Orf3a in modulating host cell responses. To achieve this, the following objectives will be pursued: (i) determine Orf3a localisation in host cells and delineate sorting motifs required for its intracellular trafficking; and (ii) elucidate the significance of Orf3a interaction with candidate host endosomal-lysosomal proteins.

Patents

Achievements

Outcome / Output

This study characterised the Orf3a accessory protein of SARS-CoV-2 and found it to localise in different endocytic compartments as well as in the Golgi compartment of the cell. It also identified key tyrosine-based sorting signal in the C-terminal tail of Orf3a responsible for its intracellular trafficking inside the host cells. Further, it was found that expression of Orf3a leads to increase in pH of lysosomes causing impairment in cargo trafficking and degradation in lysosomes.

Publications

Related Research

View All

Funding Organization

Department of Science and Technology (DST)

Quick Information

Focus Area

Therapeutics for COVID-19

Start Year

2020

Sanction Amount

₹ NA

Status

Ongoing

Contact

atuli@imtech.res.in

Output

No. of Research Paper

Technologies (If Any)

No. of PhD Produced

No. of Patents

Filed : 00

Grant : 00

Acces sibility Controls

Research Projects