Research Projects

Non-coding RNAs (ncRNAs) play an important role in DNA replication, translation, RNA splicing, and epigenetic regulation. The role of ncRNAs in tumorigenesis as either oncogenes or tumour suppressors has also been identified by the researchers. These RNAs also play an important role in initiation and progression of pancreatic cancer whose incidences are increasing year by year and is associated with high mortality. To the best of our knowledge, no nanotherapeutic systems have been developed for siRNA or AsO-based inhibition of sNHG5 lncRNA in Pancreatic Ductal Adenocarcinoma (PDAC). Therefore, the main objective of the proposed proposal is to develop and validate nanotherapeutic systems for siRNA or AsO based inhibition of sNHG5 lncRNA in Pancreatic Ductal Adenocarcinoma. Briefly, the siRNA-based Nano-systems (Lipid-based Nanoparticles) targeting sNHG10 RNA sequences will be developed and validated. The cell proliferation assay, colony formation assay, cell migration and invasion assay, cell cycle analysis, apoptotic assays, will be conducted under In-vitro conditions to check the efficacy of validated system. Further, the Co-drug-siRNA-nanotherapeutic systems will also be developed to check the synergistic effect in combination with gemcitabine. Finally, the biodistribution studies will be conducted in the experimental animals using small animal imaging system.