Research Projects

This joint research proposal aims to combine photon counting imaging technology developed by Prof. Osamu Matoba and Prof. Matoba to create a 3D fluorescence imaging technology that works under low light irradiation. This technology will enable the capture of neuronal activity in vivo without photodamage, which is crucial for understanding brain activity, disease progression, and improved diagnosis. The proposed method will use the same imaging system as the existing system, assuming a relatively large light intensity. The captured imaging dataset will be shared with Indian researchers, who will analyze the relationship between light irradiance and fluorescence imaging and generate fluorescence images with limited number of photons simulating a low light illumination environment. The proposed research will also investigate the limit of photon number against depth and develop machine learning models and advanced image processing techniques. The establishment of optical bio-imaging under low light conditions will pave the way for future observation of a wide area and stimulate the development of technology for the observation of entire brain tissue. The results will be published in high-impact journal articles and disseminated through national and international conference talks.