The project aims to develop a non-invasive ultrasound technique for detecting the sex, assessingthe gonadal maturity stages of both male and female fishes of brackishwater aquacultureimportance. The findings will help in obtaining stress free spawning without any obstruction inthe reproductive success of captive brooders. The technique to be developed will be of greathelp for private entrepreneurs to make use of user-friendly technique which will unblock thehurdles to have a strengthened scientific knowledge and facilities to start up the fin fishhatchery especially the foremost frontier of brooders development and breeding. The stress-free spawning is achieved through deep learning techniques. The ultrasound images areacquired and preprocessed using windowing, segmentation and feature extraction to removethe speckle noise which extracts texture features, shape features, histogram, correlogramfeatures and morphology features. The processed image is fed to the deep learning techniqueto identify the gonads stages which are classified into immature, mature, ripe and running.Transfer learning is used to populate more images for training the system to provide betteraccuracy. The Generative Adversarial Network (GAN) is used to classify the gonad stages. Finally,the stress-free deep learning results are compared with the histology data for validating the testresults