Plant tissue cultures are supposedly aseptic. it is now established that tissue cultures could harbour diverse bacteria in non-obvious form. Indexing procedure facilitates the detection of covert and endophytic bacteria in plant tissue cultures and to cull such cultures, thereby avoiding the risk of cross contamination from hazardous organisms. It involves a 3-step culture indexing for the detection of culturable bacteria and take timely action to maintain the cultures active and healthy.
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