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Handheld Polymerase Chain Reaction (PCR) system for DNA sequence amplification. Consists of a disposable micro fabricated amplification chip, capable of handling 20-150 µl sample volume. Micro-heater temperature is controlled using precisely designed PID controller. The system is capable of controlling the temperature cycles with precision, suitable for PCR application. Control circuitry generates the temperature/time cycles as per PCR amplification requirements.