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Typhoid is a life threatening infection caused by the Salmonella enterica serovar Typhi and currently emerging as a major public health problem in developing countries. Diagnosis of Salmonella Typhi is quite challenging because the symptoms of typhoid fever overlap with a wide range of other diseases. The conventional methods which include the blood culture and antibody based detection are considered as a gold standard for the diagnostic of typhoid fever. These methods often give rise to false negative results because of the prior intake of antibiotics and hence underestimates the actual occurrence of disease.