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Bitter gourd has been popular as a natural remedy in Ayurvedic systems owing to its anti-diabetic, anti-malarial, anti-inflammatory, antiviral, contraceptive and insecticidal properties. Bitter gourd juice (BGJ) has a rich array of bioactive phytochemicals including vitamins, minerals and phenolic compounds. However, simple preparation of juice does not extract bioactive principals to the full extent and its use is still limited due to its extremely bitter taste which limits its consumer acceptability. BARC has developed a technology for preparation of de-bittered Karela juice having high bioactivity. It is prepared by using all GRAS status chemicals and enzymes.