The invention relates to DNA markers specific to WA cytoplasmic male sterile lines of rice, for assessing seed purity and a method for ensuring the purity of cytoplasmic male sterile lines of rice using the said DNA based markers
Patents
CN1281Technology Demonstration51, US6528265
Outcome / Output
The invention relates to a method of using DNA sequences for assessing seed purity. More specifically, the invention relates to a DNA sequences having homology to rice mitochondrial DNA and being unique to Wild Abortive (WA) cytoplasm containing cytoplasmic male sterile lines of rice, and the use of these sequences in a Polymerase Chain Reaction (PCR) assay to distinguish Male Sterile (CMS) lines of rice from their cognate Male Fertile Maintainer Lines.
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