The procedures for making these novel cationic amphiphiles being simple, the prices of this new transfection reagent should be remarkably lowerthan that of LIPOFECTAMINETM and LIPOFECTIN
TM, The transfection efficiency of the novel cationic amphiphiles are much better than that of LIPOFECTINTM and comparable to or better than the transfection efficiencies ofLIPOFECTAMINETM
, The cationic amphiphiles disclosed in the present invention have been tested positive for transfecting COS 1, Hela, Vero, CV 1 and NIH3T3 cells and the primary cell line, Rat Skin Fibroblasts.